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Postranično termičko oštećenje tkiva trbušne stijenke i tankog crijeva kod štakora i svinje primjenom UltraCisiona R : doktorska disertacija

Fakulteti, doktorske disertacije Vrsta gradje
Naslov
Postranično termičko oštećenje tkiva trbušne stijenke i tankog crijeva kod štakora i svinje primjenom UltraCisiona R : doktorska disertacija / Zenon Pogorelić ; [mentor Zdravko Perko]. -
Impresum
Split : Z. Pogorelić, 2009. ([s. l. : s. n.]). -
Materijalni opis
70 listova : ilustr. u bojama, graf. prikazi (djelomice u bojama) ; 30 cm + 1 optički disk. -
Uputnica
Jezik
hrv, eng
Napomena
Na vrhu nasl. str.: Sveučilište u Splitu, Medicinski fakultet.
Bibliografija: listovi 56-60. Summary.

SUMMARY:
INTRODUCTION:
Harmonic scalpel for tissue cutting and coagulation is a replacement for the high frequency current that may cause different complications.
It is frequently used in many open and endoscopic operations.
Using Harmonic scalpel the lower amount of energy is transduced to the tissue with less possibility of the lateral thermal damage or penetration depth because of low temperatures, only 50-100o C, and also the body is free of electric passing current.
The working principle of the Harmonic scalpel is to transform the electric power into mechanical longitudinal movement of the working part of the instrument by piezoelectrical transducer situated in the hand piece.

AIM:
To determinate the effects of different employing times at application on the experimental model of rat’s and pig’s abdominal tissue and pig’s small intestine.
METHODS:
Two hundred grams weight Wistar rats were used.
The animals were etherized and anaesthetized with Ketamin with a dose of 100 ml/kg and the laparathomy was done.
Using Harmonic scalpel we performed the operation on the muscular facial part of the abdominal tissue employing different time for each group separately.
Between three and five individual approaches were done using Harmonic scalpel for each experimental group.
Forty kilograms weight pigs were used as the second experimental model.
The animals were anesthetized using the following sedation, relaxation, and narcosis regimen: ketamine 10% with a dose of 20
mg/kg, xylazine 2% in a dose of 2 mg/kg, atropine sulfate 1% in a dose of 3 mL/animal, and propofol 1% in a dose of 2–5 mL/animal.
Endotracheal anesthesia was with isoflurane (1–1.5 vol%), nitrous oxide (max. 75 vol%), and oxygen (25 vol%). After anesthesia, the animals were fixed to an operative table, and a laparotomy was performed.
The muscular part of the abdominal wall without skin or small intestine was coagulated using different application regimens for each group.
Ten individual approaches were performed for each experimental group using the Harmonic Scalpel.
After the experiment all animals were euthanized with 7,4% solution of KCl. After the animals were euthanized the tissue was cut out and inserted in paraffin.
The paraffin blocks were cut in 5µm thick pieces and colored using classic histological method hemalaun-eozin.
Under the light microscope the width of necrosis was measured from the point where the Harmonic scalpel was used.

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